Shau-Chun Wang’s Biography

Shau-Chun Wang, Professor and Director, Department of Chemistry and Biochemistry and the Center for Nano BioDetection, National Chung Cheng University

Prof. Shau-Chun (Paul) Wang is currently a professor in the Department of Chemistry and Biochemistry, National Chung Cheng University, Chia-Yi, Taiwan, where he has served as Director of the Center for Nano Bio-detection Technologies since 2013 and Director of Division of Modern Agriculture and Rural Development, Advanced Gerontological Expertise Institute since April 2017. Prof. Wang obtained his B.S. degree in Chemistry and M.S. degree in Analytical Chemistry in National Taiwan University in 1991 and 1993, respectively. He was a junior instructor in National Taiwan University 1993–1994. He obtained his PhD in Analytical Chemistry in the University of Michigan in 1999. He worked in Pfizer Global Research and Development in U.S. as a post doctoral research fellow before joined the faculty of his department in 2001. Prof. Wang was a visiting scholar to the Department of Chemical and Biomolecular Engineering, the University of Notre Dame in the summers of 2001, 2002, and 2003. Prof. Wang was awarded Young Chemist Medal by Chemical Society located in Taipei in 2008. Prof. Wang’s main research interests include electrokinetics fundamentals and their applications to develop novel microfluidic devices such as mixers, pumps, concentrators, and particle sorters for bioanalysis using spectroscopic and plamonics detections. Prof. Wang assumed a position from Feb. 2015 to July 2016 as the Deputy Provost for Research and Development of his university, in charge of technology promotion. Prof. Wang is an experienced consultant in bioanalytical development and GMP compliance for various pharmaceutical and consumer goods companies. He co-found Instant NanoBiosensors, Inc. in Aug 2016, a bioanalytical and medical diagnostics equipment R&D firm specialized in fiber optics particle plamon resonance (FOPPR) biosensors.

Binding Kinetic Constants Measurement by Using Fiber Optic Particle Plasmon Resonance Biosensor

Biosensing methods have been a popular means to measure the binding affinity and rate constants of immuno-logical reactions. In particular, the quantitative investigation of binding kinetics provides important information regarding the interactions between the docking target molecule and the counterpart probe molecule. Fiber optic particle plasmon resonance (FOPPR) biosensor, one simple and label-free sensing platform, has been successfully used to determine binding kinetic constants. The FOPPR biosensor is based on an optical fiber modified on gold nanoparticles, where the surface of gold nanoparticles has been conjugated by a mixed self-assembled monolayer (SAM) and a molecular probe reporter to dock with the corresponding analyte species. The binding process is recorded in static mode when analyte solution is loaded in one static sensing cell. The ability to analyse biomolecules without flow may be especially useful when an experiment only has a small amount of analyte available to characterize a biomolecular interaction. We compare FOPPR biosensor with commercialized Biacore 3000 SPR biosensor to determine the binding kinetic constants, which is limited by analyte injection flow rate and the probe concentration on the chip in Biacore 3000 SPR biosensor whereas FOPPR is not affected by these two effects. In addition, we established the general mathematical model to estimate the binding kinetic rate constants based on the pseudo-first-order model. We will discuss the agreement of obtained association rate constants (ka) and dissociation rate constants (kd) of anti-ovalbumin with ovalbumin and IgG binding with anti-IgG, between the computational results using our mathematical model, and the measured values.