Petr Nikitin’s Biography

Petr Nikitin, Professor and Head of Laboratory, Prokhorov General Physics Institute, Russian Academy of Sciences

Petr I. Nikitin heads the Laboratory at the Natural Science Center, General Physics Institute, Russian Academy of Sciences. His research interests include development of optical bio- and chemical sensors, magnetic biosensors and microelectronic systems, biocomputing with nanoparticles, magnetic particle quantification and mapping, as well as application of these techniques for diagnostics, food and feed control, environmental monitoring and detection of biological weapons. He received his Master’s and a Ph.D. degree (Supervisor – Nobel Prize winner A.M. Prokhorov) from Moscow Institute of Physics and Technology. He is the author of more than 150 papers published in high-ranking peer-review journals and more than 30 international patents. ?? is co-founder of Magnisense, SE com?any in Paris and Lyon, France and a member of International Advisory Committees of several major sensor conferences such as European Conference on Optical Chemical Sensors and Biosensors, European Conference on Magnetic Sensors and Actuators, etc.

Highly-Sensitive Label-Free Optical Biosensors and Multiplex Immuno-Magnetic Platform for Point-of-Care Diagnostics

Several types of original biosensors will be presented. Intelligent biosensing systems are developed based on nanoparticles that can employ molecular interactions to perform any Boolean logic function. These systems are efficiently used for logic-gated biosensing with lateral flow test-strips and 3D filters. Besides, multichannel label-free biosensors are designed based on the spectral correlation interferometry (SCI) for detection of various analytes by measuring changes in thickness of a biolayer on functionalized glass slips used as affordable single-use sensor chips. The SCI method is not sensitive to bulk refractive index of a solution under test and provides signals in metrological units (pm or nm). Using real-time monitoring of bioreactions by the SCI, a new multiplex dry-reagent immunomagnetic (DRIM) biosensing platform is developed for rapid high-precision quantitative analyses of complex mediums. It is based on the highly sensitive magnetic particle quantification (MPQ) method that provides detection limit of 60 zeptomoles or 0.4 ng of magnetic nanolabels and extremely wide linear dynamic range of 7 orders. The DRIM platform has permitted the detection in human serum of as low as 25 pg/ml total prostate specific antigen during 30-min assay. The featured 3-fold signal increase per every order of concentration within 3.5 orders of magnitude allows precise analysis of antigen concentrations in a wide range. In addition, based on the MPQ technique, a new cytometry method has been developed and successfully tested for rapid assessment of cancer HER2/neu status of cells, for estimation of expression level of various antigens on cell surfaces and cancer diagnostics.